Contact Info

Adam P. Hitchcock

Canada Research Chair

in Materials Research
CLS-CCRS
B.I.M.R
McMaster University
Hamilton, ON
Canada L8S 4M1
V: +1 905 525-9140
    x24729
F: +1 905 521-2773
E: aph@mcmaster.ca
U: unicorn.mcmaster.ca
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Fluorescence Imaging of 2-component supported lipid bilayers

WHO:  Jonathan West, Jose moran-Mirabal, Adam Hitchcock,
                  Chemistry &Chemical Biology, BIMR, McMaster University

WHERE:  McMaster Universty, ABB-103
 
WHEN:   2016                        POSTED:  22 Jun 2016

WHAT: Using Fluorescence Microscopy to Optimize phase               segregation in mixed lipid bilayer

As part of his M.Sc. project to study mechanisms of interaction of cationic antimicrobial peptides and bacterial cell membranes, Jon West developed methods to prepare supported lipid bilayers (SBL) with 2 different lipid components, intended for later study using STXM. The figure shows 6 time points in the development of the phase segregated SLB as a 25:75 mol% mixture of 1,2-di-(9Z-octadecenoyl)-sn-glycero3-phosphocholine (DOPC) and 1,2-dioctadecanoyl-sn-glycero-3-phosphocholine (DSPC) as the
mixture slowly cools from an initially homogeneous solution at 70 C.

OTHER INFORMATION: A movie of the DOPC-25 / DSPC-75 phase segregation can be viewed here

2016 A.P. Hitchcock / McMaster University - All Rights Reserved
web site design by Christopher Amis (2002). Last updated on 22 Jun 2016 (aph)